Verification of determination of phosphate (PO43-) method in domestic wastewater using the ascorbic acid method with uv-vis spectrophotometer

Qonita Lufiana; Moh. Syaiful Arief; Aman Sentosa Panggabean

doi:10.32698/GCS-SNIIBIPD3446

Verification of determination of phosphate (PO43-) method in domestic wastewater using the ascorbic acid method with uv-vis spectrophotometer

Qonita Lufiana⁽¹⁾, Moh. Syaiful Arief⁽²⁾, Aman Sentosa Panggabean^(3*)

(1) Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman, Samarinda, Indonesia
(2) Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman, Samarinda, Indonesia
(3) Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman, Samarinda, Indonesia
(*) Corresponding Author

Abstract

The research about the verification of determination of phosphate (PO43-) method in domestic wastewater using the ascorbic acid method with UV-Vis spectrophotometer at PT Global Environment Laboratory Samarinda has been done. The determination of phosphate (PO43-) in domestic wastewater samples refers to SNI 06-6989.31-2005. Some important parameters about the verification of the measurement method such as: determination of the value of correlation coefficient measurement, precision, accuracy, LOD and LOQ has been carried out. The result of the research shows that the linearity of the measurement is very good, shown with the correlation coefficient value was 0.9976. The LOD and LOQ values were 0.0281 mg/L and 0.1221 mg/L, respectively. The precision of this method shown as coefficient variance of Horwitz was 3.1242% and the accuracy of the research as a recovery percentage was 89.2-102.68%, indicating that the ascorbic acid method can be used for analysis of phosphate (PO43-) in domestic wastewater.

Keywords

phosphate (PO43-); ascorbic acid; verification method

References

Adha, L.S., Panggabean, A.S. dan Kartika, R. 2017. Optimasi Kinerja Analitik Terhadap Penentuan Kadar Fluorin Pada Batubara Dengan Metode Spektrofotometer Visible, Jurnal Atomik, 2(1). Hal. 143-145.
Destiana, L.V., Panggabean, A.S., dan Kartika, R. 2017. Pengembangan Metode Rapid Test Preparation Dalam Penentuan Kadar Inherent Moisture Dan Total Sulfur Dengan Metode Yang Dipergunakan Oleh ISO (International Organization For Standardization, Jurnal Atomik, 2(1). Hal. 175-182.
Harmita, 2004. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian. 1(3).Hal. 117-135.
Kusuma, E.A.S., Panggabean, A.S. dan Arafat, Y. 2015. Optimasi Kinerja Analitik Pada Penentuan Kadar Fosfor Sebagai P2O5 Pada Abu Batubara Dengan Metode Spektrofotometer Visible, Jurnal Kimia Mulawarman, 13(1). Hal. 9-14.
Kusumaningtyas, D.I. dan Purnama, P. 2017. Analisa Kadar Fosfat (P-PO4) di Perairan Sungai Citarum Dan Anak Sungainya Dengan Metode Asam Askorbat, Buletin Teknik Litkayasa, 15(1). Hal.23-27.
Napitupulu, R.M., Julia, D., dan Panggabean, A.S. 2019. Validasi Metode Penentuan Mn Dalam Oli Lubrikan Dengan Metode Pengenceran Langsung Menggunakan Spektrofotometer Serapan Atom, Indo. J. Chem. Res., 6(2). Hal. 36-42.
Panggabean, A. S, Pasaribu, S. P, and Kristiana, F. 2018. The Utilization of Nitrogen Gas a Carrier Gas in Determination of Hg Ions Using Cold Vapor-Atomic Absorption Spectrophotometer (CV-AAS). Indones. J. Chem. 18 (2). pp. 279-285.
Panggabean, A.S., Widyastuti, T. dan N Hindryawati, N. 2019. Validasi Metode Penentuan Benzena, Toluena dan Xilena pada Sampel Udara dan Tanah Menggunakan Kromatografi Gas, ALCHEMY Jurnal Penelitian Kimia 15(1).Hal. 177-189.
Peraturan Pemerintah Republik Indonesia No. 82 Tahun 2001 Tentang Pengelolaan Kualitas Air dan Pengendalian Pencemaran Air.
Miller, J.N., and Miller, J.C., 2005. Statistics and Chemometrics for Analytical Chemistry. 5th Edition. Pearson Education Limited, England.
Riyanto. 2014. Validasi & Verifikasi Metode Uji Sesuai dengan ISO/IEC 17025 Laboratorium Pengujian dan Kalibrasi, Deepublish, Yogyakarta.
Sugiharto. 2008. Dasar-Dasar Pengelolaan Air Limbah. Jakarta: UI-Press.

Proceeding:

Seminar Nasional 1 Baristand Industri Padang

Publisher:

Redwhite Press

ISSN (Print):

2685-5984

ISSN (Electronic):

2685-5933

Published:

December 2020